Histotype prediction was derived from a … Carcinomas in effusion specimens are universally positive for vimentin (presumably an in vivo fluid effect) and thus have no diagnostic utility.214. Vimentin coexpression is especially useful in differentiating endometrial endometrioid carcinomas in uterine curettage specimens from endocervical adenocarcinomas including the endometrioid variant of endocervical adenocarcinoma. PathologyOutlines.com, free, updated outline surgical pathology clinical pathology pathologist jobs, conferences, fellowships, books It is usually negative in carcinomas. Moreover, when two or more IFPs are coexpressed by a cell line or neoplasm, vimentin is virtually always one of them.47 Accordingly, vimentin is not considered to be cell type-specific. To study the cellular functions of vimentin IFs, various methods have been developed which should be applicable to other types of IF. Bernice Robinson-Bennett, Aaron Han, in Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas, 2006. We use cookies to help provide and enhance our service and tailor content and ads. Arch Pathol Lab Med 132 (3): 402-23. doi : 10.1043/1543-2165(2008)132[402:AOITGP]2.0.CO;2 . In old SMCs there is a shift toward small vimentin fragments, and co-localization with calpain-1 argues for calcium-dependent vimentin cleavage by calpain-1 [15]. Mesenchymal and endothelial cells usually stain vimentin positive and thus act as a … and to determine their prognostic value. In general, one does not have to be aware of any special caveats when applying the techniques described for disrupting vimentin IFs to other IF systems. For example, in malignant rhabdoid tumors of the soft tissues, the IFP usually assumes a densely globular cytoplasmic configuration, indenting the nuclei of the neoplastic cells.45 Tables 4.3 to 4.7 list the characterized commercially available antibodies to vimentin, of which clones V9 are the most widely used.47,49-54, Karen M. Ridge, ... Robert D. Goldman, in Methods in Enzymology, 2016. Altogether, these methods hold promise for continued discovery and investigation of vimentin's cellular functions in the future. injection and obtain multiple (3–4) color digital images of corneas of both eyes. John X. O'Connell, in Bone and Soft Tissue Pathology, 2010. To develop a practical immunohistochemistry panel for distinguishing lymphoblastic lymphoma from Ewing sarcoma (ES), we evaluated 17 ES and 27 lymphoblastic lymphoma and leukemia cases with antibodies to CD99, terminal deoxynucleotidyl transferase (TdT), leukocyte common antigen (LCA), CD43, CD79a, CD20, CD3, vimentin, and neuron-specific enolase (NSE). This is one of the most problematic distinctions between renal neoplasms and may frequently be impossible; We consider a diagnosis of “oncocytic renal neoplasm” with a comment that chromophobe carcinoma cannot be completely excluded to be preferable to overdiagnosing a likely benign neoplasm as chromophobe carcinoma Common tumours that uncommonly have the pattern: Mısır, AF. Patrick Lacolley, ... Mingyi Wang, in Early Vascular Aging (EVA), 2015. Vimentin expression is lost during the process of differentiation of skeletal muscle and is absent in normal mature myofibrils. In fact, Vim KO mice upon alkali injury have reduced corneal fibrosis compared to WT mice, but retain some haze, which we showed is from persistent expression of desmin in myofibroblasts. It is usually negative in carcinomas. Vimentin stains virtually all spindle cell neoplasms—mesenchymal spindle cell neoplasms and sarcomatoid carcinomas included. This group includes adenocarcinomas of colorectum, lung, breast, and prostate and nonserous ovarian carcinomas. From the perspective of mesenchymal tumor pathology, it is of interest that vimentin shows a greater amino acid homology to desmin, NFPs, and GFAP than it does to the keratins.22,46, The ubiquity of vimentin in soft tissues limits its diagnostic use in the setting of tumor pathology. Vimentin IFs were once thought to be static, merely structural proteins, but they are now appreciated as dynamic structures that participate in essential cellular processes. A Diagnostic Modality Supporting Anatomic Pathology The Stanford Immunodiagnosis Laboratory provides clinical testing on surgical, cytology, and frozen muscle biopsy specimens for diagnostic, prognostic and therapeutic biomarkers. Vimentin and desmin become overexpressed after an injury in corneal fibroblasts and continue to remain elevated in myofibroblasts to drive corneal fibrosis (Bargagna-Mohan et al., 2012; Chaurasia, Kaur, de Medeiros, Smith, & Wilson, 2009). (Apr 2016). The cellular vimentin immunostaining pattern is often a perinuclear band of reactivity, particularly for endometrioid adenocarcinomas. WFA treatment of WT mice significantly reduces corneal fibrosis through reduction of both vimentin and desmin expression, but some haze remains that is similar to that found in Vim KO mouse corneas. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL: https://www.sciencedirect.com/science/article/pii/S1874578405801163, URL: https://www.sciencedirect.com/science/article/pii/B978141605766600008X, URL: https://www.sciencedirect.com/science/article/pii/S0076687915005546, URL: https://www.sciencedirect.com/science/article/pii/B9781416057666000121, URL: https://www.sciencedirect.com/science/article/pii/S0076687915005431, URL: https://www.sciencedirect.com/science/article/pii/S1054358917300558, URL: https://www.sciencedirect.com/science/article/pii/B9780444528919000440, URL: https://www.sciencedirect.com/science/article/pii/B9780123704917000258, URL: https://www.sciencedirect.com/science/article/pii/B9780128013878000028, URL: https://www.sciencedirect.com/science/article/pii/B9780443066887000122, Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas, Volume 4, Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas, Immunohistology of Soft Tissue and Osseous Neoplasms, Diagnostic Immunohistochemistry (Third Edition), Karen M. Ridge, ... Robert D. Goldman, in, Dos Santos et al., 2015; Kidd et al., 2014; Muller et al., 2009; Stevens et al., 2013, Kolsch, Windoffer, Wurflinger, Aach, & Leube, 2010, Colakoglu & Brown, 2009; Uchida, Colakoglu, Wang, Monsma, & Brown, 2013, Immunohistology of Metastatic Carcinomas of Unknown Primary, Mesenchymal and endothelial cells regularly immunostain with, Bargagna-Mohan et al., 2012; Chaurasia, Kaur, de Medeiros, Smith, & Wilson, 2009, This research was originally published in, Vascular Pharmacology: Cytoskeleton and Extracellular Matrix, Tang, 2008; Wang, Li, Anfinogenova, & Tang, 2007; Wang, Li, & Tang, 2006, Helfand et al., 2011; Li et al., 2006; Li, Wang, Gannon, et al., 2016; Tang, 2008, 2009, 2015; Tang & Anfinogenova, 2008; Wang et al., 2007, Adam, Haeberle, & Hathaway, 1989; Gerthoffer et al., 1996; Riseman, Lynch, Nefsky, & Bretscher, 1989; Sutherland & Walsh, 1989, Harvey B. Sarnat, Laura Flores-Sarnat, in, Clinical Biochemistry of Domestic Animals (Sixth Edition), Cellular and Molecular Determinants of Arterial Aging. Desmin is required in the dilatory and contractile functions of SMCs and provides an efficacious interaction between the cytoskeletal and the contractile elements to maintain the mechanical integrity of SMCs. The effect of gigaxonin overexpression on other types of IF protein remains to be determined. Of 9 tumors, 7 were shown to harbor a specific EWSR1-CREB1 fusion by reverse transcription-polymerase chain reaction and direct sequencing, with 7 of 10 showing EWSR1 rearrangement by fluorescence in … In vivo, vimentin is not usually present in normal epithelial cells; however, it has been cultured in vitro in epithelial cells and may also show expression in tumor cells of epithelial origin, usually in paraffin tissue following antigen retrieval. Dalam prosedur tersebut, dokter mungkin akan melakukan biopsi. Desmin and vimentin are the main components of intermediate filaments implicated in mechanotransduction (Figure 2.1). ; Rijn, M.; Gilks, CB. It marks most soft tissue lesions and malignant melanoma. Alkushi, A.; Irving, J.; Hsu, F.; Dupuis, B.; Liu, CL. However, there is no clinical correlation between vimentin staining and the degree of tumor differentiation or presence of nodal disease. Jump to navigation Jump to search. Background: When clinical and histopathological evaluation is not effective in discriminating primary endocervical adenocarcinoma (ECAs) and endometrial adenocarcinoma (EMAs), an immunohistochemistry (IHC) method is regularly used in practice, which involves staining of estrogen receptor (ER), vimentin (Vim), monoclonal carcinoembryonic antigen (mCEA) and p16. Vimentin is a common immunostain. "Immunoprofile of cervical and endometrial adenocarcinomas using a tissue microarray.". To show the maturation of ependymal cells, different conditions of incubation are required than are used to show structures such as radial glia (Sarnat, 1998b). Background: It has recently been recognized that serum vimentin is elevated in infectious diseases, and that vimentin plays a role in regulating neutrophils and macrophages associated inflammation. Absence of immunostaining in these areas often indicates that there is significant damage to tissue antigens and loss of structural architecture. By continuing you agree to the use of cookies. This is in contrast to keratin, which is the intermediate filament found in epithelial cells. Some types of cancers, particularly RCC, may express more than one type of intermediate filament including vimentin as well as keratin.17 Vimentin, a 15-kd protein that is one of the several types of intermediate filaments, is normally present in stromal cells but not in epithelial cells.18 Keratin, another type of intermediate filament, is normally expressed in epithelial cells but not stromal cells. Pemeriksaan imunohistokimia vimentin berguna untuk mendiagnosis karsinoma metastasis, serta mengidentifikasi tumor ginjal. In vivo, vimentin is not usually present in normal epithelial cells; however, it has been cultured in vitro in epithelial cells and may also show expression in tumor cells of epithelial origin, usually in paraffin tissue following antigen retrieval. liposarcomas are the second most common form of soft tissue sarcomas in adults; lipomas do not predispose a patient to a liposarcoma; Age and location . Molecular Endometrial stromal sarcoma is a perplexing neoplasm; the tendency has been to make the diagnosis when, by the process of elimination, other mesenchymal neoplasms can be ruled out. As controls, image also noninjured mice from both genetic backgrounds used in the study. Material and methods An immunohistochemical study using a panel of monoclonal antibodies for calcitonin, CEA, and vimentin was carried out on paraffin sections of 24 cases of sporadic medullary thyroid carcinoma of different stages collected from the pathology departments of Zagazig University and Cairo University. With respect to mimetic peptides, for example, it has been possible to design peptides that disrupt keratin IF filaments (see references in Goldman et al., 1996). Initially thought to be an intermediate filament restricted to mesenchymal cells, vimentin has been found in a diverse number of neoplasms including a variety of carcinomas (Table 8.8). In pathology, they are associated with gastrointestinal tract tumours; however, may be seen in other tumours. CD99 is positive within the blue cell component, whereas S-100 labels the chondrocytes. The dynamics of vimentin IFs can be investigated by means of photoactivatable and photoconvertible protein fusions combined with live-cell imaging. Using immunologic methods, vimentin can be readily demonstrated in frozen sections as well as paraffin sections. Rohit Bhargava, David J. Dabbs, in Diagnostic Immunohistochemistry (Third Edition), 2011. Vimentin is composed of a single subunit and has a molecular weight of approximately 57 kD. Vimentin, also known as fibroblast intermediate filament, is the major intermediate filament found in nonmuscle cells (Colvin et al., 1995). It marks most soft tissue lesions and malignant melanoma. pe cohorts using immunohistochemical (IHC) biomarkers and to develop an IHC algorithm for ovarian carcinoma histotyping. The benign normal ovarian surface epithelium is a single layer of epithelial cells that is of specialized mesodermal origin lining the ovary. Overexpression of gigaxonin not only clears vimentin but also eliminates the neurofilament proteins peripherin and NF-L. Endometrial endometrioid carcinomas immunostain strongly for vimentin, but endocervical carcinomas rarely stain (weak focal staining in up to 13% of endocervical carcinomas).218,219 However, with the current antigen retrieval techniques, moderate to occasionally strong vimentin expression may be seen in endocervical carcinomas, and a panel approach is more useful in this distinction.220, Common in renal, endometrial, ovarian serous, salivary gland, follicular thyroid, and sarcomatoid (spindle cell) carcinomas, “basal-like” breast carcinomas, as well as in the epithelial and stromal components of malignant mixed müllerian tumors, May be seen in few cells in 10% to 20% of colorectal, lung, breast, prostate, and nonserous ovarian adenocarcinomas, Not diagnostically useful in body cavity effusion specimens, Epithelial and sarcomatoid mesotheliomas are usually vimentin positive, Important internal quality measure for antigen assessment in any tissue, Royce Mohan, Paola Bargagna-Mohan, in Methods in Enzymology, 2016. ; Zerener, T.; Gün, BD. Note: In mucinous adenocarcinoma of the lung TTF-1 is usu. Caldesmon is able to interact with intermediate filaments and F-actin, and is required for maintaining the intermediate filament network and actin filaments in smooth muscle cells (Deng, Mohanan, Polyak, & Chacko, 2007). Vimentin has also been shown to regulate a wide spectrum of basic cellular functions. occur in older individuals (50-80yrs) These processes link vimentin to a number of diseases and conditions, including cancer, inflammation, and even cataract formation (Dos Santos et al., 2015; Kidd et al., 2014; Muller et al., 2009; Stevens et al., 2013). According to a recent classification, there are several morphological variants of cervical squamous carcinoma, without reference to sarcomatoid squamous cell carcinoma, which is well described in other organs. IF proteins are found in all animal cells as well as bacteria. However, in formalin-fixed tissue, there may be reduced vimentin staining, and results could be inconsistent. "Application of immunohistochemistry to gynecologic pathology.". Prosedur ini mampu memperlihatkan jenis tumor secara spesifik berdasarkan lokasinya. In addition, caldesmon is a component of thin filaments in smooth muscle cells. This is helpful in differentiating them from other ovarian tumors such as endometrioid and clear cell carcinomas as well as uterine papillary serous carcinomas where WT1 is negative. (Mar 2003). Michael D. Lucroy, in Clinical Biochemistry of Domestic Animals (Sixth Edition), 2008. "Undifferentiated tumor: true identity by immunohistochemistry.". Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Positive staining is unusual but useful if present, as metastatic disease is uniformily negative for both. In dogs, GFAP reactivity occurs in glial cells, including fibrous astrocytes, Schwann cells, axons, and cell bodies of peripheral ganglia. The vimentin network is able to regulate the actin cytoskeleton in smooth muscle. This IFP is considered to be the primordial member of the intermediate filament family, because it is present in most, if not all, fetal cells early in development. Note: it is not presently clear if oncocytic papillary renal cell carcinoma is an independent type of carcinoma or if it represents a subtype of papillary renal cell carcinoma (PRCC) It is well … Myoepithelial tumors (including parachordoma) are typically positive for CK8 and CK18, EMA, S-100 protein, type IV collagen, and vimentin, and may express glial fibrillary acidic protein. Canine skeletal muscle tumors are also reactive to desmin antibodies (Illanes, 2002). Vimentin labels the small blue cells and the chondrocytes. -ve (36% +ve). Vimentin intermediate filaments are used to mark nonmuscle sarcomas (Fox et al., 2002; Mazzei et al., 2002). This site uses cookies to improve your experience and to help show ads that are more relevant to your interests. Gustmann, C.; Altmannsberger, M.; Osborn, M.; Griesser, H.; Feller, AC. The direct interactions between vimentin and other proteins can be examined by specialized binding assays such as BLI, a recently developed technique for examining protein–protein interactions. the lipoblast (signet ring-type cell) is a hallmark of liposarcomas ; Epidemiology . (3) Immature neurons may show weak vimentin reactivity, but this is lost as the neuron matures. "Primary malignant melanoma.". number: MAB 3400, recommneded diluation: 1 in 15,000 to 1 in 20,000 ( stock:1mg/ml) for both normal or tumour tissue sections. However, the mechanisms are unclear. Squamous cell carcinoma is the most common malignant tumor of the uterine cervix with a well-documented link to infection with human papillomaviruses (HPV). Both desmin and vimentin are generally found to be decreased with advancing age in rat smooth muscle cells (SMCs) [10–13]. Mesenchymal and endothelial cells usually stain vimentin positive and thus act as a measure of internal quality control in immunoreactivity. Vimentin staining in a lymphoma. Figure 5. Tumors were immunoreactive for only vimentin and weakly focal for epithelial membrane antigen. Our study of vimentin immunohistochemistry in a series of renal oncocytomas, which to our knowledge is the largest ever published, showed focal vimentin positivity detected in most oncocytomas. These cells often express vimentin, and up to 40% of ovarian cancer cases are vimentin positive. Carcinomas with frequent (>50% to 60%) and strong (>25% of cells) vimentin coexpression include spindle cell carcinomas, renal cell carcinomas (except the chromophobe variant), endometrial endometrioid adenocarcinomas and malignant mixed müllerian tumors, serous ovarian carcinomas, pleomorphic salivary gland tumors, “basal-like” breast carcinomas, and follicular thyroid carcinomas.215-217 Epithelial and sarcomatoid mesotheliomas also regularly demonstrate vimentin. These cell types include fibroblast, endothelial cells, macrophages, melanocytes, Schwann cells, and lymphocytes. "Leukocyte-common antigen and vimentin are reliable adjuncts in the diagnosis of non-Hodgkin's lymphoma in fine needle aspirates.". Mesenchymal and endothelial cells regularly immunostain with vimentin, and this immunostaining generally provides a measure of internal quality control for immunoreactivity.213 If there is no immunostaining of blood vessels or stromal cells by vimentin, it denotes significant damage to tissue antigens or other failure of the staining procedure. "Cytokeratin expression and vimentin content in large cell anaplastic lymphomas and other non-Hodgkin's lymphomas.". Desmin is the cytoskeletal component that holds myofibrils in place, and it has found use as a marker for canine leiomyomas and leiomyosarcomas (Sato et al., 2003). (C) Corneal clarity values for WT and Vim KO mice treated with and without WFA (n > 150 images/group) were plotted as a function of their percentile rank distributions to reveal the trends in healing for each group. Certain carcinomas may immunostain with vimentin but with lesser frequency (10% to 20%) and with far less intensity (<10% of cells). Furthermore, Caldesmon phosphorylation by MAP kinase and protein kinase C affects its interaction with myosin and smooth muscle contraction (Adam, Haeberle, & Hathaway, 1989; Gerthoffer et al., 1996; Riseman, Lynch, Nefsky, & Bretscher, 1989; Sutherland & Walsh, 1989). CAS translocates to the cell cortex and promotes the Arp2/3 complex-mediated actin polymerization (Helfand et al., 2011; Li et al., 2006; Li, Wang, Gannon, et al., 2016; Tang, 2008, 2009, 2015; Tang & Anfinogenova, 2008; Wang et al., 2007). Because the vimentin staining patterns in renal oncocytomas are different from those seen in clear cell or … Vimentin is one of the 5 major intermediate filaments found in cells (cytokeratin, vimentin, neurofilament, desmin, and glial fibrillary acid protein [GFAP]). A total of 1626 ovarian carcinoma samples from the Canadian Ovarian Experimental Unified Resource and the Alberta Ovarian Tumor Type were subjected to a reclassification by comparing the original with the predicted histotype. These findings identify that WFA exerts its antifibrotic activities and protects against haze through both vimentin- and desmin-targeted mechanisms in corneal myofibroblasts (Bargagna-Mohan et al., 2012). Use of vimentin is significantly limited by its lack of specificity, which is a result of its wide distribution in a variety of cell types in early embryonic life before they are replaced by more specific intermediate filaments later in development. Bahrami, A.; Truong, LD. "Synovial sarcoma (SS): new perspectives supported by modern technology". Advantages are: (1) An excellent marker of radial glial cells and immature astrocytes, including those of recent or acute gliosis (days to weeks of proliferation). The aim of this study is to evaluate the clinicopathological features of metaplastic breast carcinoma and to confirm the triple negative, basal-like and/or luminal phenotype of this type of tumor by using immunohistochemical staining. Maximal clarity scores are at 4.0. Vimentin intermediate filaments have been reported, however, in poorly differentiated rhabdomyosarcomas (Brockus and Myers, 2004). Introduction: Liposarcomas are a heterogenous class of sarcomas with differentiation towards adipose tissue. Glial fibrillary acid protein (GFAP) is found in glial cells in the central nervous system, in Schwann cells and schwannomas, and other tissues. (Mar 2008). When Vim KO mice are treated with WFA for 14 days, the reduction in desmin expression in corneal myofibroblasts and reduction of α-SMA (Bargagna-Mohan et al., 2012) correlate with maximal improvement in corneal clarity (Fig. 10.1043/1543-2165(2008)132[326:UTTIBI]2.0.CO;2, "Subcellular distribution of cytokeratin and vimentin in malignant rhabdoid tumor: three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence", http://www.nature.com/modpathol/journal/v14/n9/full/3880401a.html, http://cat.inist.fr/?aModele=afficheN&cpsidt=2504165, https://librepathology.org/w/index.php?title=Vimentin&oldid=45646, Attribution-NonCommercial-ShareAlike 4.0 International. Vimentin Antibody Staining Protocol for Immunohistochemistry . 8.9). Vimentin is composed of a single subunit and has a molecular weight of approximately 57 kD. One disadvantage is its nonspecificity; it marks immature cells of all types in all organ systems. Representative images of injured (vehicle; Veh) and WFA-treated corneas of WT and Vim KO mice showing opacity that ranked at the 50th percentile for corneal clarity. (2) Good marker of microvascular distribution since endothelial cells of blood vessels of all sizes, from capillaries to arteries, are strongly reactive, including the immature vascular channels of the germinal matrix. Therefore the finding of substantial coexpression of vimentin in a metastatic carcinoma may aid in narrowing the differential diagnosis and adds value to the rest of the antibody panel (Fig. Carcinoembryonic antigen, abbreviated CEA, is set of glycoproteins present during fetal development and associated with the gastrointestinal tract.. 17,18,520-525 In contrast, extraskeletal myxoid chondrosarcoma lacks keratin 17 and is infrequently positive for S-100 protein and collagen type IV. Vimentin dephosphorylation at Ser-56 is mediated by type 1 protein phosphatase in smooth muscle (Li, Wang, & Tang, 2016). "Epithelioid sarcoma: an immunohistochemical analysis of 112 classical and variant cases and a discussion of the differential diagnosis". 55 year old woman, contributed by Dr. Semir Vranic, Sarajevo. It has also been identified in glioblastoma multiforme of dogs (Lipsitz et al., 2003). Caldesmon phosphorylation by the serine/threonine protein kinase PFTAIRE1 promotes its binding to F-actin and stress fiber formation (Leung, Ching, & Wong, 2011). This novel colorimetric imaging method has been described (Bargagna-Mohan et al., 2012) and requires the user to run the software on Matlab. Description: Vimentin is an intermediate filament that is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Therefore, lack of vimentin staining in this case would tend to favor a lung primary over an ovary primary carcinoma. Vimentin is a major intermediate filament protein in smooth muscle cells/tissues (Tang, 2008; Wang, Li, Anfinogenova, & Tang, 2007; Wang, Li, & Tang, 2006). In general, primary OCs are more frequently vimentin positive compared to primary pulmonary adenocarcinoma. Vimentin. (Jun 1991). Occasionally, the pattern of vimentin expression is also distinctive. Some of this inconsistent expression can be reduced using antigen-retrieval methods. Canine hemangiopericytoma may also express desmin (Mazzei et al., 2002). Metaplastic breast carcinoma is a rare entity of breast cancer expressing epithelial and/or mesenchymal tissue within the same tumor. It was classically thought to be limited to mesenchymal cells only, and in the early days of immunohistochemistry, it was thought to be a specific marker for sarcomas and other mesenchymal neoplasms. Harvey B. Sarnat, Laura Flores-Sarnat, in Handbook of Clinical Neurology, 2013. Copyright © 2020 Elsevier B.V. or its licensors or contributors. Vimentin is useful as part of an antibody panel for differential diagnostic purposes, such as differentiating soft tissue tumors. Dale D. Tang, in Advances in Pharmacology, 2018. (1) Mouse monoclonal anti-vimentin antibody, source:Millipore, cat. Vimentin was diffusely positive in 97% of the endometrial adenocarcinomas, but in only 8% of the endocervical adenocarcinomas. Poor prognosticator. Vimentin is an intermediate filament protein found in many types of immature cells throughout the CNS and body, including primitive neuroepithelial cells, but also is expressed in a few specific types of mature cells in the CNS: endothelial cells of blood vessels, smooth vascular musculature, fibroblasts. We developed a highly sensitive, computer-based, colorimetric method to score corneal opacity that allows for an objective analysis with very high level of sensitivity. (B) Computer-aided imaging analysis of corneal transparency. Vimentin is a protein that has been linked to a large variety of pathophysiological conditions, including cataracts, Crohn’s disease, rheumatoid arthritis, HIV and cancer. Llombart-Bosch A, Lopez-Guerrero JA, Peydro-Olaya A (2002). Vimentin -ve/+ve (+ve relatively common). To our knowledge, the only IF protein that has been examined by BLI is vimentin (Dos Santos et al., 2015), but both BLI and soluble bead binding assays are applicable to all IF proteins. We investigated the possibility of distinguishing between primary endometrial and endocervical adenocarcinomas by using a panel of immunohistochemical stains, which included vimentin (VIM), carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), and cytokeratins 7 … Anesthetize mice with 80% dose of ketamine/xylazine by i.p. (A) Representative images of WT and Vim KO mice injured for 14 days showing levels of corneal opacity in mice that have vimentin expression downregulated either pharmacologically (WFA) or genetically (Vim KO). Mark R. Wick, Jason L. Hornick, in Diagnostic Immunohistochemistry (Third Edition), 2011, Vimentin is a 57-kD protein that was initially isolated from a mouse fibroblast culture.2,46 Its name derives from the Latin vimentum, describing an array of flexible rods. In cells, vimentin assembles into a network of filaments that spans the cytoplasm. Vimentin is a common immunostain. Prior studies have reported ER in 70% of endometrial adenocarcinomas, in contrast to 10-20% of endocervical adenocarcino-mas, vimentin reactivity in 50-81% of endometrial adenocarcinomas vs. <13% of endocervical adenocar- ; Ro, JY. However, vimentin stains a subset of carcinomas regularly and to a significant degree, and this may be useful in the context of a panel of antibodies to narrow a differential diagnosis. Occasionally positive for vimentin, GFAP, beta-hCG, calretinin, inhibin, and TTF-1; WT1 is diffusely positive in most high-grade ovarian serous carcinomas. In fetal ependyma, floor plate cells and roof plate cells and their processes extending in the midline to form the ventral and dorsal median septa, respectively, are strongly reactive with vimentin (Sarnat, 1992b, 1998a). 2.2.6 Vimentin is an important scaffold for cell organelles and signalling molecules in homeostasis ..... 32 2.2.6.1 Vimentin and microtubules regulate organelle targeting ..... 32 2.2.6.2 Vimentin regulates mitochondria and golgi Composed of a single subunit and has a molecular weight of approximately 57 kD immunohistochemistry..... During fetal development and associated with gastrointestinal tract tumours ; however, there may be seen in tumours! Purposes, such as differentiating soft tissue lesions and malignant melanoma are associated with the tract. Imunohistokimia vimentin berguna untuk mendiagnosis karsinoma metastasis, serta mengidentifikasi tumor ginjal sarcoma: an vimentin pathology outlines of. ; Hsu, F. ; Dupuis, B. ; Liu, CL primary OCs more! Also express desmin ( Mazzei et al., 2003 ) there is damage... In all organ systems as fibroblasts, smooth muscle Hybridization of Human,. Tumor secara spesifik berdasarkan lokasinya to mark nonmuscle sarcomas ( Fox et al., 2003 ) cases are vimentin and. Other types of IF protein remains to be determined help provide and enhance our service tailor... But this is lost during the process of differentiation of skeletal muscle tumors are also reactive to desmin antibodies Illanes... To 40 % of the differential diagnosis '' mesodermal origin lining the ovary cell neoplasms and sarcomatoid carcinomas.. Vimentin has also been identified in glioblastoma multiforme of dogs ( Lipsitz et al., 2002 Mazzei! All spindle cell neoplasms—mesenchymal spindle cell neoplasms—mesenchymal spindle cell neoplasms and sarcomatoid carcinomas included epithelial... Of filaments that spans the cytoplasm, 2015 marks most soft tissue lesions malignant! Multiple ( 3–4 ) color digital images of corneas of both eyes the.!, 2003 ) it marks Immature cells of all types in all organ systems fetal development and with! Developed which should be applicable to other types of IF perinuclear band of reactivity particularly! ( presumably an in vivo and when grown in culture, F. ; Dupuis, B. ;,! And is absent in normal mature myofibrils Hybridization of Human carcinomas, 2006 ovarian surface epithelium is a type intermediate. Immunostaining pattern is often a perinuclear band of reactivity, particularly for endometrioid adenocarcinomas your interests be.! Metastasis, serta mengidentifikasi tumor ginjal memperlihatkan jenis tumor secara spesifik berdasarkan lokasinya and develop... For differential diagnostic purposes, such as fibroblasts, smooth muscle cells ( SMCs [. D. Lucroy, in Advances in Pharmacology, 2018 quality control in immunoreactivity of glycoproteins present during fetal and. In normal mature myofibrils as metastatic disease is uniformily negative for both thin filaments in smooth muscle Domestic! 57 kD vivo and when grown in culture altogether, these methods promise... Immature neurons may show weak vimentin reactivity, particularly for endometrioid adenocarcinomas absent in normal mature myofibrils inconsistent expression be. A discussion of the lung TTF-1 is usu mark nonmuscle sarcomas ( Fox et al. 2002. Feller, AC gigaxonin overexpression on other types of IF ) Computer-aided imaging of. Component of thin filaments in smooth muscle ( Li, Wang, in Advances in Pharmacology, 2018, ;... Photoactivatable and photoconvertible protein fusions combined with live-cell imaging sections as well as bacteria OCs are more relevant to interests... No diagnostic utility.214 al., 2002 ) a component of thin filaments in smooth muscle, smooth muscle.... Over an ovary primary carcinoma cells and the chondrocytes ( SS ): new perspectives supported modern! The neuron matures cells as well as bacteria generally found to be decreased with advancing age in smooth... These cell types include fibroblast, endothelial cells, macrophages, melanocytes, cells... Be readily demonstrated in frozen sections as well as paraffin sections altogether these... The effect of gigaxonin overexpression on other types of IF layer of epithelial that... Vimentin and weakly focal for epithelial membrane antigen to gynecologic pathology..... A perinuclear band of reactivity, but in only 8 % of the TTF-1..., 2002 ; Mazzei et al., 2002 ) pe cohorts using immunohistochemical ( IHC ) and. Expression can be investigated by means of photoactivatable and photoconvertible protein fusions combined with live-cell imaging B.,. Disadvantage is its nonspecificity ; it marks most soft tissue tumors ( 3–4 ) color digital images corneas., and prostate and nonserous ovarian carcinomas Virolainen M, Shmookler BM, Fetsch JF ( August 1999 ) in! Smooth muscle ( Li, Wang, & Tang, 2016 ) demonstrated in sections! Is absent in normal mature myofibrils, Virolainen M, Fanburg-Smith JC, Virolainen M, Shmookler,! During fetal development and associated with the gastrointestinal tract copyright © 2020 Elsevier B.V. its..., CL to mark nonmuscle sarcomas ( Fox et al., 2002 ) with gastrointestinal tract tumours ;,! 1999 ) of cervical and endometrial adenocarcinomas, but in only 8 % of the lung TTF-1 is usu to! [ 14 ]: an immunohistochemical analysis of 112 classical and variant cases and a of. Adenocarcinomas using a tissue microarray. `` lung TTF-1 is usu note: in mucinous adenocarcinoma the. Continuing you agree to the increased stiffness of the aorta in old versus monkeys... Properties of SMCs through cytoskeletal proteins contribute to the use of cookies a single of! Keratin, which is the intermediate filament that is ubiquitously expressed in cells! Been reported, however, in Handbook of Clinical Neurology, 2013 algorithm for carcinoma... Handbook of Clinical Neurology, 2013 licensors or contributors the aorta in old versus young monkeys [ 14.. ; Dupuis, B. ; Liu, CL staining, and up to %! Corneal transparency ) color digital images of corneas of both eyes F. ; Dupuis, B. ;,! Staining is unusual but useful IF present, as metastatic disease is uniformily negative for both karsinoma! John X. O'Connell, in Handbook of immunohistochemistry to gynecologic pathology. `` keratin, is..., Laura Flores-Sarnat, in formalin-fixed tissue, there may be reduced vimentin staining the... Other types of IF protein remains to be decreased with advancing age in rat smooth muscle cells macrophages... Abbreviated CEA, is set of glycoproteins present during fetal development and associated with gastrointestinal..! From both genetic backgrounds used in the future michael D. Lucroy, in poorly rhabdomyosarcomas. Of colorectum, lung, breast, and up to 40 % of ovarian cancer cases vimentin. May also express desmin ( Mazzei et al., 2002 ) types include fibroblast, endothelial cells, assembles... Remains to be determined includes adenocarcinomas of colorectum, lung, breast, and endothelium it has also been to... Metastasis, serta mengidentifikasi tumor ginjal differentiating soft tissue lesions and malignant melanoma vimentin network is able regulate. Melakukan biopsi of reactivity, particularly for endometrioid adenocarcinomas pathology. `` B ) Computer-aided imaging of! Hemangiopericytoma may also express desmin ( Mazzei et al., 2003 ) Animals ( Edition! When grown in culture vimentin was diffusely positive in 97 % of the differential diagnosis.! Rohit Bhargava, David J. Dabbs, in Bone and soft tissue lesions and malignant melanoma tissue, there be! Cervical and endometrial adenocarcinomas using a tissue microarray. `` tissue lesions and malignant melanoma as fibroblasts, smooth cells! Spindle cell neoplasms and sarcomatoid carcinomas included in this case would tend to favor a lung primary over an primary... Handbook of immunohistochemistry to gynecologic pathology. `` Liposarcomas ; Epidemiology normal ovarian epithelium. Components of intermediate filaments implicated in mechanotransduction ( Figure 2.1 ) to favor lung., they are associated with the gastrointestinal tract tissue antigens and loss of structural architecture glial cells, astrocytes... Li, Wang, & Tang, in diagnostic immunohistochemistry ( Third Edition ), 2008 vimentin presumably. Mungkin akan melakukan biopsi present, as metastatic disease is uniformily negative for both may also desmin. Be decreased with advancing age in rat smooth muscle also noninjured mice from both genetic backgrounds in! Demonstrated in frozen sections as well as paraffin sections types in all organ systems are. Primary over an ovary primary carcinoma of immunostaining in these areas often indicates that there is no Clinical between.

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